The authors also performed studies to optimize the process. A decrease in catechin and theaflavin gallates and a significant increase in ga content and the relative. High performance liquid chromatography and metabolomics. Elbanna department of food science and technology, faculty of agriculture, alexandria university, alexandria. Aspergillus niger was grown in a selective medium and then genomic dna was directly extracted from fungal mycelium. Tannase production in submerged fermentation by aspergillus sp. Isolation and screening of tannase producing fungi. The optimum conditions for maximum tannase enzyme production in the submerged fermentation smf using aspergillus niger was. Aspergillus niger gh1 tannase selfimmobilized during the ssf process. This fungus strain was cultivated in an erlenmeyer flask under a submerged fermentation system. Evaluation of culture conditions for tannase production by. The culture supernatants obtained by filtration through whatman no. Tannase production by aspergillus niger aa20 was studied in submerged smf and solidstate ssf fermentation systems with different tannic acid and glucose concentrations.
Research on conditions of solidstate fermentation and. Production, characterization and purification of tannase from aspergillus niger article pdf available in european journal of experimental biology 25. Optimized production of tannase from cashew testa using aspergillus niger mtcc 5898. Optimization of tannase production by aspergillus niger in solidstate packedbed bioreactor article pdf available in journal of microbiology and biotechnology 219. Selection of tannaseproducing aspergillus niger strains. Tannase is an enzyme widely used in the food industry, mainly for wine and beer production. Production of gallic acid using the immobilized cells of aspergillus oryzae has been studied. Extracellular tannase production was induced when the fungus was cultured in khanna medium with tannic acid as carbon. Some of these fungal strains such as aspergillus niger gh1 and psh strain have demonstrated to be excellent enzyme producers. Bioconversion of naturally occurring tannins into a value added pharmaceutical intermediate gallic acid a new approach h. Culture conditions for the production of a tannase of aspergillus tamarii imi388810. Abstract gallic acid 3,4,5 trihydrixybenzoic acid is an organinc substance occuring in many plants either as a free molecule or as part of tannic acid molecule. Gallic acid production by immobilization of aspergillus orzae. Among the different temperatures such as 25, 30, 35, 40 and 45c tried, the maximum enzyme production was observed at 35c 48 ugmin.
Gallic acid production with mouldy polyurethane particles. Rhizophora apiculata bark is a tanninrich waste material obtained from charcoal industry. Growth of colonies in plates with tannic acidcontaining medium indicated their ability to synthesize tannase. Zumrut ogel august 2006, 90 pages in this study, a filamentous fungus, aspergillus niger was evaluated in terms of extracellular tannase production. Production of tannase using aspergillus niger by submerged. Lower or higher agitation speeds than 200 rpm produced lower enzyme production and fungal growth. To demonstrate that fungal tannase excretion is strongly influenced by the culture conditions, tannase production by aspergillus niger gh1 on smc using different bioreactors was evaluated. A method for assay of microbial tannase tannin acyl hydrolase based on the formation of chromogen between gallic acid and rhodanine is reported.
Accordingly, four isolates, which exhibited high tannase activity, were chosen to. The production of tannase by aspergillus niger gh1 in solidstate fermentation ssf was evaluated. The most active tannase producer was selected and identified as aspergillus niger aumc 4301 at mycological center, faculty of science, assuit university, egypt. Culture conditions for the production of a tannase of aspergillus tamarii imi388810 b enemuor, s. Tannase production by aspergillus niger 194 was sterilized and inoculated with 2% vv inoculum was added and incubated at 35 c for 36 h in a rotary shaker 200rpm. Study is on the solidstate fermentation of tannase with the gallnut by aspergillus niger b0201. Aspergillus niger holds the generally recognized as safe status from the food and drug authority and is the primary. Optimized production of tannase from cashew testa using aspergillus niger mtcc 5898 article pdf available in food biotechnology 304. Some factors affecting tannase production by aspergillus niger van tieghem hamada a.
Optimisation of fermentation conditions for production of. Using designed primers, pcr carried out and a 950bp expected band obtained that was ligated into cloning vector and then cloned into escherichia coli top10f in. Effect of agitation speed on morphological changes in. College, nanded 431601, india corresponding author abstract introduction gallic acid, a phenolic compound, chemically known as 3,4,5. Pdf original article statistical optimization for tannase. Production of tannase from aspergillus niger under solid state fermentation zainab saadi mahdi, shatha salman altahan department of biology, college of science, university of baghdad, baghdad, iraq abstract thirteen a. Although tannase production by aspergillus niger can occur in the absence of tannic acid, this fungus tolerates tannic acid concentrations as high as 20%wv, without having a deleterious effect on both the growth and. Production cost of enzymes, the major constraint in bioprocessing, may be brought down by multifaceted approaches which include the use of. The aim of this work was to select strains of aspergillus niger for tannase production. Aspergillus niger isolate which was proved efficient was used for gallic acid production. Tannase activity was also measured in solidstate fermentation. Biosynthesis and characterization of aspergillus niger. Central composite rotatable design ccrd falling under response surface methodology rsm was used. Identification, isolation, cloning and sequencing of tannase.
Culture conditions for the production of a tannase of. An extracellular tannase was produced from solidstate cultures of aspergillus niger. Maximum tannase production occurred in the culture broth containing 12% wv tannic acid and 0. Statistically based experimental design was employed for the optimization of fermentation conditions for maximum production of enzyme tannase from aspergillus niger. The current study investigated the production of tannase by aspergillus niger cepc 11 mtcc 5898 on an abundantly available waste byproduct cashew testa under solid state a. The production of enzymes by bioprocessing is a good alternative to add value to agroindustry residues. Preliminary culture conditions for aspergillus nigergh1. In this regard there are no reports on bacterial tannase production using tannin containing any agrobased substrates. Effects of initial substrate tannic acid concentration, incubation time and temperature on tannase production in ssf have been studied. Addition of high tannic acid concentrations increased total tannase activity in ssf, while in smf it was decreased.
Identification, isolation, cloning and sequencing of. Based on the sem and tem micrograph observation, there was. Various physical parameters were studied in order to maximize the tannase production. The experiments involved in the process and conditions of solidstate fermentation and factors influencing characters of tannase. Original article statistical optimization for tannase production from aspergillus niger under submerged fermentationr. Agitation speed fungal hyphae aspergillus niger tannase introduction the economics of a process. The optimal agitation speed was at 200 rpm which produced 1. Sdspage analysis as well as gel localization studies of purified tannase indicated the presence of two enzyme forms, with molecular. Production, characterization and purification of tannase from.
Central composite rotatable design ccrd falling under response surface methodology. One variable at a time procedure was used to evaluate the effect of qualitative variables on the production of tannase from aspergillus niger van tieghem. However, the diversity of fungi stays poorly explored for tannase production. Bioconversion of naturally occurring tannins into a value. The results indicated that the unpasteurized method before fermentation was in favor of the higher enzymic activity than the sterilized method. For tannase production by paecilomyces variotii, temperature range of 4065 c and ph range of 4. Pdf optimized production of tannase from cashew testa. Optimization of gallic acid production from terminalia chebula 291 from the fig. Involvement of physical parameters in medium improvement. The ph, incubation period, temperature and glucose concentration optima of tannase production was found at 5. Aspergillus niger fbt1, a local extracellular strain for tannase production, was isolated from soil collected from matang mangrove forest reserve in perak, malaysia. There is a significant effect p tannase from fungal sources is reported to be highly active over a wide range of ph and temperature. Aspergillus niger growth on polyphenolic carbon sources and optimization of the tannase production souad akroum department of applied microbiology and food sciences, faculty of natural sciences, university of jijel, ouled aissa city, jijel 18000, algeria. Pdf optimization of tannase production by aspergillus.
Although tannase production by aspergillus niger can occur in the absence of tannic acid, this fungus tolerates tannic acid concentrations as high as 20%wv, without having a deleterious effect on both the growth and enzyme production. These properties make the enzyme suitable for pollution control and. Production, characterization and purification of tannase. Production of tannase by rhizopus oryzae and aspergillus foetidus from the powdered fruits of terminalia chebula and caesalpinia digyna has been reported11. Agitation speed was found to influence the tannase production and fungal growth of aspergillus niger fetl ft3. This study was carried out to optimize the physical parameters for the maximal tannase production by aspergillus niger via solid. Abstract aspergillus niger is a mould known to be the best tannase producer. Characterization of a thermostable extracellular tannase. This industrial waste was used as solid substrate in the study for the production of tannase and at the same time, help in minimizing the countrys industrial wastes. Gallic acid formation from gallotanninsrich agricultural. Aspergillus niger mtcc 3557 was used for maximum production of tannase enzyme and the factors affecting the production were optimized. Extra and intracellular tannase production by aspergillus niger gh1 has been evaluated using submerged smf and solidstate fermentation ssf at different temperatures 30, 40 and 50 c.
Nowadays, tannase is produce by submerged culture smc being the enzyme obtained in an intracellular form resulting, as a consequence, in high production costs. In this work, we measured tannase in aspergillus niger fermented tea leaves and confirmed the production of fungal tannase during puerh tea fermentation. Opadanje aktivnosti tanaze nakon 20 sati ugoja povezano je s pratecim porastom aktivnosti proteaze. The present study explores the natural ability of fungi to utilize tannin as a source of carbon. A study on optimization of submerged fermentative production of tannase by aspergillus niger was carried out.
Influence of agitation speed on tannase production and. The optimum values of parameters obtained through response surface methodology rsm were cashew testa 23%, k. Statistical optimization for tannase production from. Sep 27, 2011 agitation speed was found to influence the tannase production and fungal growth of aspergillus niger fetl ft3.
The optimum temperature for the enzyme activity was found to be 3040c, at which the enzyme activity was. Tannase is also utilized for bioremediation of effluents from tanneries. A tannase from aspergillus niger was used for esterification of catechin using gallic acid in ionic liquids, a process known as galloylation raab et al. Tannase from fungal sources is reported to be highly active over a wide range of ph and temperature. Optimized production of tannase from cashew testa using. Production of tannase from aspergillus niger under solid. Thermostable tannase from aspergillus niger application in. Purification, characterization and application of tannase. Process parameters influencing tannase production by. Accepted april, 2009 aspergillus tamarii imi388810 b a tannic acid degrading fungus was isolated from soil inundated by effluent of a tannery at oji river local government area of enugu state, nigeria. Economic production of tannase by aspergillus niger van tiegh. Pdf selection of tannase producing aspergillus niger strains.
Economic production of tannase by aspergillus niger van tiegh adopting different fermentation protocols and possible applications romanian biotechnological letters, vol. Optimization of gallic acid production from terminalia. This study was carried out to optimize the physical parameters for the maximal tannase production by aspergillus niger via solid substrate fermentation using a shake flask system. Bioconversion of naturally occurring tannins into a value added pharmaceutical intermediate gallic acid a new approach. This fungus was cultivated in 250 ml erlenmeyer flask under submerged fermentation system. The enzyme was purified to homogeneity from the cellfree culture broth by preparative isoelectric focusing and by fplc using anionexchange and gelfiltration chromatography. In addition, tannase is used as a sensitive analytical probe for determining the structure of naturally occurring gallic acid ester. Materials and methods microorganism and culture medium the microorganism used for tannase production, a.
Some factors affecting tannase production by aspergillus. Gallic acid production with mouldy polyurethane particles obtained from solid state culture of aspergillus. To study the effect of increasing doses of gamma radiation on tannase production by a. Economic production of tannase by aspergillus niger van. Aspergillus niger fetl ft3, a local extracellular tannase producer strain that was isolated from one of dumping sites of tanninrich barks of rhizophora apiculata in perak, malaysia. Pdf optimization of tannase production by aspergillus niger. Aspergillus niger is cultured for the industrial production of many substances. Production of tannase by aspergillus niger ozturk, alev deniz m.
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